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bacteria
bacteria
SciBear: Bearing the knowledge

WE ANALYZE
MICROBIAL GENOMES

The SciBear team analyzes all types of microbial genome sequencing data. We provide efficient, fast and reliable analysis of your data. Our specialized SciBear analysis report is designed to be easily incorporated into scientific manuscripts.
PRODUCTS
SERVICES WE OFFER:
New microbial species are discovered almost every day. High-quality assembly and annotation of the microbial genome is the basis for successful future research. SciBear has developed a pipeline for de novo assembly and annotation of microbial genomes.
Raw DNA sequencing reads are the only input we need from you. We perform a quality check on your DNA reads, assemble the microbial genome and analyze it for completeness and potential contamination. We will determine the taxonomic position of the assembled genome and its average nucleotide identity (ANI) to the nearest microbial genome. We will annotate the genome, evaluate CRISPRs, and analyze COGs and Pfam domains. Your SciBear report will provide results that meet the Minimum Information about the Genome Sequence (MIGS) specification (doi: 10.1038/nbt1360).

De novo assembly and annotation of microbial genomes

genome assembly
For the study of microbial communities, SciBear has developed a pipeline for microbiome analysis using a metagenomic approach. When genetic material has been extracted directly from environmental or host-associated samples, metagenomic analysis is required to explore the microbial communities.

The composition of the microbial community and its functional potential can be determined based on the microbial DNA recovered from the samples. Raw DNA sequencing reads are the only input we need from you. After a quality check and read cleaning, we assemble and annotate the metagenome to determine the metabolic capabilities of the analyzed community.

To reconstruct the microbial genomes (Metagenome-Assembled Genomes or MAGs) of a given sample, we perform binning of the metagenome using at least three different methods.

Metagenomic analysis of microbiomes

metagenomic analysis
Pan-genomic analysis is the latest approach to decipher the entire genetic content of a set of genomes. Pan-genomic analysis represents the entire genetic variability of a group of organisms. Pan-genomic analysis can be used to find homologous genes and predict the metabolic properties of a microorganism.

Pan-genomic analysis reveals the diverse genomic features that explain a range of microbial adaptations to different environments. Pan-genomic analysis is essential for proper taxonomy or reclassification of species.

We are happy to help you with the pan-genomic analysis of microorganisms of your interest. To perform this analysis, we only need information about the group of microbes whose genomes you want to compare and which metabolic pathways are of particular interest.

Microbial pan-genome analysis

pan-genome analysis
Evolutionary relationships between different microbial species can be explained by phylogenetic analysis. Phylogenetic characterization is critical for contextualizing genomes without prior phenotypic information, and for determining their genetic novelty and genotype-phenotype relationships.

SciBear's team has extensive experience in both reclassifying existing and describing new microbial taxa, as evidenced by publications in peer-reviewed journals such as the International Journal of Systematic and Evolutionary Microbiology (IJSEM) and Bergey's Manual of Systematics of Archaea and Bacteria (BMSAB). We are happy to share our experience and help you with your phylogenetic analysis. The object of analysis can be phylogenetic markers (e.g. 16S rRNA), concatenated single-copy core genes of analyzed genomes, or any gene or group of genes of interest.

Microbial phylogenetics

microbial phylogenetic
Send us your data
Now relax and let us do the work
Your results!
As soon as we have a clear idea of which output format you want, we'll request your raw data to get started. Your sequencing results can be shared with us in any way convenient for you (for example, through a link to Dropbox, by FTP, etc.)

Your report will be generated within 3 days! While you wait, you can plan your next research project and think about big scientific ideas.
Your results will be delivered in the form of a FASTA file containing your genome assembly, a brief report with the main outcomes, and a detailed report (you can see a sample report here)
HOW DOES IT WORK?
Just fill in the form with the key information about your request, and we will reply to you as soon as possible. Feel free to ask any questions by e-mail to: info@sci-bear.com
Just press the "order now" button and fill in the form

WHAT OUR CLIENTS SAY ABOUT US
PhD in Biology (Lomonosov Moscow State University)

Specialist in microbial genomics, systematics, and evolution. Co-author on more than 100 publications in peer-reviewed scientific journals.

Master of Applied Mathematics and Informatics (Moscow Institute of Physics and Technology)

Bioinformatics analyst, software engineer. Co-author on more than 20 publications in peer-reviewed scientific journals.

Master of Science in Biology (Lomonosov Moscow State University)

Biologist, specialist in project management. Co-author of 5 patents.

DENIS GROUZDEV
MARIA KRUTKINA
KATERYNA PANTIUKH
TEAM
KIRILL LOBANOV
Master of Science in Finance and Supply Chain Management (Plekhanov Russian University of Economics)

Sourcing Professional with 7 years of experience in supply chain practices for different groups of commodities.

OUR NEWS

    HBC Kleikamp, D Grouzdev, P Schaasberg, R van Valderen, R van der Zwaan, R van de Wijgaart, Y Lin, B Abbas, M Pronk, MCM van Loosdrecht, M Pabst
    Metaproteomics, metagenomics and 16S rRNA sequencing provide different perspectives on the aerobic granular sludge microbiome
    Water Research. 2023; 246, 120700. doi:10.1016/j.watres.2023.120700

    Y Boltyanskaya, T Zhilina, D Grouzdev, E Detkova, N Pimenov, V Kevbrin
    Halanaerobium polyolivorans sp. nov.—a novel halophilic alkalitolerant bacterium capable of polyol degradation: physiological properties and genomic insights
    Microorganisms. 2023; 11 (9), 2325. doi: 10.3390/microorganisms11092325

    AP Ershov, TL Babich, DS Grouzdev, DS Sokolova, EM Semenova, AN Avtukh, AB Poltaraus, EA Ianutsevich, TN Nazina
    Genome analysis and potential ecological functions of members of the genus Ensifer from subsurface environments and description of Ensifer oleiphilus sp. nov.
    Microorganisms. 2023; 11 (9), 2314. doi:10.3390/microorganisms11092314

    S Parshina, E Zhuravleva, AA Nikitina, D Grouzdev, N Kostrikina, V Kevbrin, A Novikov, D Kopitsyn, T Kolganova, R Baslerov, AN Nozhevnikova, Yu Litti
    Syntrophic growth of Biomaibacter acetigenes strain SP2 on lactate and glycerol
    Fermentation. 2023; 9 (6), 557. doi:10.3390/fermentation9060557

    YV Boltyanskaya, VV Kevbrin, DS Grouzdev, EN Detkova, VV Koziaeva, AA Novikov, TN Zhilina
    Halonatronomonas betaini gen. nov., sp. nov., a haloalkaliphilic isolate from soda lake capable of betaine degradation and proposal of Halarsenatibacteraceae fam. nov. and Halothermotrichaceae fam. nov. within the order Halanaerobiales
    Systematic and Applied Microbiology. 2023; 46 (3), 126407. doi:10.1016/j.syapm.2023.126407

    VV Koziaeva, DY Sorokin, TV Kolganova, DS Grouzdev
    Magnetospirillum sulfuroxidans sp. nov., capable of sulfur-dependent lithoautotrophy and a taxonomic reevaluation of the order Rhodospirillales
    Systematic and Applied Microbiology. 2023; 46 (3), 126406. doi:10.1016/j.syapm.2023.126406

    V Ryzhov, V Deriglazov, D Grouzdev, V Koziaeva, I Kiselev, I Larionov, K Gareev, N Sitkov, T Zimina, Y Marchenko, M Shevtsov
    Biogenic nanomagnetic carriers derived from magnetotactic bacteria: magnetic parameters of magnetosomes inside Magnetospirillum spp.
    Applied Sciences. 2023; 13(4):2431. doi:10.3390/app13042431

    I Kravchenko, D Grouzdev, M Sukhacheva, T Minayeva, A Sirin
    Microbial communities of peaty permafrost tundra soils along the gradient of environmental conditions and anthropogenic disturbance in Pechora river delta in the Eastern European Arctic
    Diversity. 2023; 15(2):251. doi:10.3390/d15020251

    M Uzun, V Koziaeva, M Dziuba, L Alekseeva, M Krutkina, M Sukhacheva, R Baslerov, D Grouzdev
    Recovery and genome reconstruction of novel magnetotactic Elusimicrobiota from bog soil
    The ISME Journal, 2023 17 (2), 204-214. doi:10.1038/s41396-022-01339-z

    AA Nikitina, AY Kallistova, DS Grouzdev, TV Kolganova, AA Kovalev, DA Kovalev, V Panchenko, I Zekker, AN Nozhevnikova, YV Litti
    Syntrophic butyrate-oxidizing consortium mitigates acetate inhibition through a shift from acetoclastic to hydrogenotrophic methanogenesis and alleviates VFA Stress in thermophilic anaerobic digestion
    Applied Sciences. 2023; 13(1):173. doi:10.3390/app13010173